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Keep those Escobarias warm and humid, Cohan Very few cacti need cold stratification and most are extremely easy to germinate, but contrary to all expectations they don't like direct sun and dry soil for the first few months of life. I start all cacti under plastic and artificial lights and I keep them that way for about 4 months, longer for very slow growing species. Escobarias are tougher than some, but still can't take full sun until at least six months old.One exception is the very alpine cactus Maihuenia poeppegii. Cold stratification seems to help with germination, although simple aging also seems to break the dormancy. It also enjoys high light early in life although it should still be kept moist. Possibly quite a few people have this one in their alpine collection.
OK,I'm not inferring any taxonomic significance here. You may note that in the first picture several of the seedlings have produced a first true leaf (which I have not observed in the other two species - generally cotyledons only in year one).Also note the red stems. Whilst we are familiar with biflora having red flowers it varies considerably in the wild from yellow through bronze/copper to red. The picture here shows the underside of the cotyledons which vary from green to solid red and I wonder if this will correlate with the degree of red pigment in the eventual flowers?I can't tell the other two apart at this stage, however the true leaves of tschernjaewii are very distinctive in year two. See the second picture. It's leaf is simply is not as divided and carrot-like as those of the other species, merely gently lobed, and retains this characteristic into adulthood.
For me Anemone biflora sown in autumn, germinated in spring after snowmelt. They went dormant over summer and rested that way until the start of this month here. Mine are 1 and 2 years old- so fingers crossed they get to flowering size.